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Antibodies against electronegative LDL inhibit atherosclerosis in LDLr-/- mice BJMBR
Grosso,D.M.; Ferderbar,S.; Wanschel,A.C.B.A.; Krieger,M.H.; Higushi,M.L.; Abdalla,D.S.P..
In order to determine the effect of antibodies against electronegative low-density lipoprotein LDL(-) on atherogenesis, five groups of LDL low receptor-deficient (LDLr-/-) mice (6 per group) were immunized with the following antibodies (100 µg each): mouse anti-LDL(-) monoclonal IgG2b, rabbit anti-LDL(-) polyclonal IgG or its Fab fragments and mouse irrelevant monoclonal IgG and non-immunized controls. Antibodies were administered intravenously one week before starting the hypercholesterolemic diet (1.25% cholesterol) and then every week for 21 days. The passive immunization with anti-LDL(-) monoclonal IgG2b, polyclonal antibody and its derived Fab significantly reduced the cross-sectional area of atherosclerotic lesions at the aortic root of LDLr-/- mice...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Minimally modified LDL; Passive immunization; Monoclonal antibody; Atherogenesis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200007
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Endocrine profiles and ovulation rate of cows superovulated with FSH following passive immunization against steroid free-bovine follicular fluid Braz. J. Vet. Res. Anim. Sci.
ALVAREZ,Rafael Herrera; CARVALHO,João Batista Pereira de; ROSA E SILVA,Alzira; PERONE,Cibele Nunes; RIBELA,Maria Tereza Carvalho Pinto; OLIVEIRA FILHO,Enoch Borges de.
Ten multiparous non-lactating cows were randomly assigned to one of two groups. On days 8 to 12 after estrus, the first group was given 100 ml antiserum against steroid-free bovine follicular fluid (anti-bFF), which was produced in ovariectomized sheep. The second group (control) was given 100 ml nonimmune ovariectomized sheep serum. Six hours after injection, both groups were superovulated with FSH (18 NIH-FSH-S1 units) and LH (0.29 NIH-LH-S1 units) in a 4-day decreasing regime. On the morning of the third day a luteolytic dose of prostaglandin F2alpha analogue (cloprostenol) was given. Artificial inseminations (AI) were done 48 and 60 h later. Embryos were collected 7 days after AI by a nonsurgical method. Blood samples were taken throughout the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superovulation; Follicular fluid; Passive immunization; Embryos; Cattle.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000600007
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Placental transfer of Haemophilus influenzae type b antibodies in malnourished pregnant women BJID
Cavalcante,Rejane S.; Kopelman,Benjamin I.; Costa-Carvalho,Beatriz T..
This study evaluated the vaccination response to Haemophilus influenzae type b (Hib) in malnourished pregnant women (MN), cord blood (CB) and in infants at two and six months of age for comparison with a control group (C). Twenty-eight malnourished pregnant women and 29 pregnant controls were immunized with conjugated Act-HIB® in the third trimester of pregnancy. Blood samples were collected from all before the immunization, during labor (post immunization), and from CB. All infants were immunized with Hib vaccine according to normal vaccine schedule and sera were collected at two and six months of age. Antibody levels to polyribosylribitol phosphate (PRP) were similar for both groups. Preimmunization: MN 1.94 µg/mL, C 1.68 µg/mL; post-vaccination: MN...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pregnant women; Malnourished; Haemophilus influenzae type b; Passive immunization; Vaccine; Antibodies; Immunoglobulin G.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100011
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